1)
Look at the control cell on the bottom to check
for non-reactivity. If it is non reactive continue.
2)
Find the first cell line with no reactivity on
the right
3)
Cross off all antibodies on the top except the
following pairs
C |
c |
E |
e |
M |
N |
S |
s |
Fya |
Fyb |
Jka |
Jkb |
4)
If any of the cells listed in step 3 are positive
without the cell to the right or left being positive it
can be crossed out.
a.
If C,c or E,e have not yet been eliminated:
i.
If D is negative, antigens C and c are on the red
cell are both positive and the cell line in non-reactive
then C and c can be ruled out.
ii.
If D is negative, antigens E and e are on the red
cell are both positive and the cell line in non-reactive
then C and c can be ruled out.
5)
Look at the remaining antigens that have not been
crossed out.
a.
Find a cell line that is positive for that
antigen but non reactive on the right
b.
Exclude all possible antigens this way including
those that are listed in step 3.
c.
If necessary use the reactions from the
additional cells on the bottom
6)
Look and see which antigen pairs have not been
excluded. If any of the following have not been
excluded, they are listed as unlikely based on the fact
that they are very rare.